home
***
CD-ROM
|
disk
|
FTP
|
other
***
search
/
Shareware Overload Trio 2
/
Shareware Overload Trio Volume 2 (Chestnut CD-ROM).ISO
/
dir26
/
med9410b.zip
/
M94A0266.TXT
< prev
next >
Wrap
Text File
|
1994-10-08
|
3KB
|
47 lines
Document 0266
DOCN M94A0266
TI Studies of the mechanism of cytolysis by HIV-1-specific CD4+ human CTL
clones induced by candidate AIDS vaccines.
DT 9412
AU Miskovsky EP; Liu AY; Pavlat W; Viveen R; Stanhope PE; Finzi D; Fox WM
3rd; Hruban RH; Podack ER; Siliciano RF; Department of Medicine, Johns
Hopkins University School of; Medicine, Baltimore, MD 21205.
SO J Immunol. 1994 Sep 15;153(6):2787-99. Unique Identifier : AIDSLINE
MED/94358445
AB Vaccine-induced, virus-specific CTLs may rapidly eliminate the host
cells that first become infected after virus exposure, thereby
preventing disseminated infection. Thus, there is much interest in the
ability of candidate AIDS vaccines to elicit CTLs. All HIV-1 envelope
(env) protein-based vaccines tested to date in seronegative humans
induce CTLs from the CD4+ subset. Because the mechanism of cytolysis by
CD4+ CTLs is controversial, a detailed study of the cytolytic reactions
mediated by vaccine-induced, HIV-1-specific human CD4+ CTL clones was
conducted. CD4+ CTL clones induced rapid destruction of Ag-pulsed target
cells. Lysis was readily detectable within 15 min. Lysis was not a
result of syncytium formation between CD4+ effector cells and
env-expressing targets. Target cell destruction was not dependent upon
de novo RNA or protein synthesis in either the effector or the target
cell. Expression of perforin mRNA was detected by Northern blotting and
reverse-transcriptase-PCR in CD4+ CTL clones but not in autologous B
lymphoblastoid cell lines. Immunohistochemical studies demonstrated
perforin protein in cytoplasmic granules in CD4+ CTL clones. Lysis by
CD4+ CTLs was strictly dependent upon extracellular Ca2+ and was highly
specific, with no lysis of innocent bystander cells. DNA fragmentation
was detectable in target cells, but did not precede 51Cr release. Taken
together, these results provide a dramatically different view of
cytolysis by human CD4+ CTLs. Target cells are lysed by a rapid and
efficient mechanism that involves a preformed mediator and that is
functionally similar to the mechanism used by CD8+ CTLs.
DE Apoptosis/IMMUNOLOGY AIDS Vaccines/*IMMUNOLOGY Base Sequence
Calcium/PHYSIOLOGY Cell Line Clone Cells Cytotoxicity Tests,
Immunologic Gene Products, env/IMMUNOLOGY Giant Cells/IMMUNOLOGY
Human HIV-1/*IMMUNOLOGY Immunohistochemistry Membrane
Glycoproteins/BIOSYNTHESIS Molecular Sequence Data Polymerase Chain
Reaction Protein Precursors/IMMUNOLOGY Support, U.S. Gov't, P.H.S.
T-Lymphocytes, Cytotoxic/*IMMUNOLOGY T4 Lymphocytes/*IMMUNOLOGY
JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).